Microvascular anatomy of olfactory and accessory olfactory (vomeronasal) organs in adult Xenopus laevis: scanning electron microscopy of vascular corrosion casts / Anatomía microvascular de órganos olfatorios y accesorios (vomeronasal) en adultos Xenopus laevis: microscopía electrónica de barrido de moldes de corrosión vascular

Microvascular anatomy and histomorphology of olfactory and vomeronasal organs in adult Xenopus laevis Daudin were studied by scanning electron microscopy of vascular corrosion casts and paraplast embedded stained serial tissue sections. Results show that the arterial supply is bilaterally by terminal arterioles of the medial branch of the nasal artery and by the palatal artery. Arterioles give rise to a capillary meshwork characteristic for respiratory surfaces in principal chambers and in dorsal and caudal areas of middle chambers. Anterior and inferior areas of the middle chambers own a distinctly different capillary network with conspicuous short capillary loops. Loops have a dilated tip and extend in acute angles towards the chamber lumen. The vomeronasal organ (VNO) locates beneath the olfactory organ. It has a medial to lateral extension and attaches with its caudal circumference to the medial nasal glands. Its capillary bed displays rectangular meshes which preferentially orientate along the long axis of the VNO. Locally, capillaries form short hairpin-like or strongly twisted loops with dilated tips which point towards the lumen of the VNO. These capillaries slow-down blood velocity and may lead to an increased exchange of oxygen, nutrients and water-borne odorants in the middle chambers and of pheromones in the VNO. In the latter vascular structures are present which might serve as a vascular pump.

Se estudiaron la anatomía microvascular e histomorfología de los órganos olfatorios y vomeronasales de Xenopus laevis Daudin adultos, mediante microscopía electrónica de barrido de moldes de corrosión vascular y secciones de tejido seriadas, teñidas e incluídas en paraplast. Los resultados muestran que el suministro arterial es bilateral por arteriolas terminales de la rama medial de la arteria nasal y por la arteria palatina. Las arteriolas dan lugar a un lecho capilar característico de las superficies respiratorias en las cámaras principales y en las áreas dorsal y caudal de las cámaras intermedias. Las áreas anterior e inferior de las cámaras centrales poseen una red capilar significativamente diferente con llamativos bucles capilares cortos. Los bucles tienen una punta dilatada y se extienden en ángulos agudos hacia la luz de la cámara. El órgano vomeronasal (VNO) se ubica debajo del órgano olfatorio. Se extiende de medial a lateral y se une con su circunferencia caudal a las glándulas nasales mediales. El lecho capilar muestra mallas rectangulares que se orientan preferentemente a lo largo del eje longitudinal del VNO. Localmente, los capilares forman bucles cortos en forma de horquilla o fuertemente retorcidos con puntas dilatadas que apuntan hacia la luz del VNO. Estos capilares ralentizan la velocidad de la sangre y pueden conducir a un mayor intercambio de oxígeno, nutrientes y odorizantes, a base de agua en las cámaras intermedias y de feromonas, en el VNO. En este último, están presentes estructuras vasculares que podrían servir como una bomba vascular.

Corrosion casting, a known technique for the study and teaching of vascular and duct structure in anatomy / Inyección corrosión, una técnica conocida para el estudio y enseñanza de ductos y estructuras vasculares en anatomía

Teaching and learning anatomy, as a process, has changed. Fresh cadavers were once used as a tool for the student to approach the human body in order to overcome theoretical knowledge and gain applied expertise. Today, techniques such as corrosion casting are known to be a more effective way of achieving optimal results with the students. This paper examines a method to apply this technique to an organ using different polymers. The concentrations for acrylic, epoxy resin, polyester resin, and room temperature vulcanization (RTV) silicone are described, as well as the corresponding diameter of the duct to be injected with each one. A variety of specimens obtained using this technique, their qualities and characteristics are presented. The results of using these procedures while involving the students, showed increased sense of responsibility, dedication and awareness, which led them to take the class more seriously and enjoy the process of learning. Additionally, the specimens left by the students will help future classes reduce the number of specimens needed.

El proceso de aprendizaje y enseñanza en anatomía ha cambiado recientemente, el cadáver fue la primera herramienta que permitía al estudiante una aproximación practica al cuerpo humano, facilitando que este se sobrepusiera al conocimiento teórico adquirido en el aula, y desarrollara experticia aplicada. Hoy en día, técnicas como la inyección corrosión son conocidas por lograr mejores resultados con los estudiantes. Este trabajo presenta un método para aplicar esta técnica a un órgano, mediante el uso de diferentes polímeros. Las concentraciones adecuadas de acrílico, resina epoxica, resina poliéster, y silicona RTV (room temperature vulcanization) son descritas, así como el diámetro sugerido para el uso de cada polímero. Se presenta una variedad de especímenes obtenidos mediante esta técnica, así como sus cualidades y características. Al integrar a los estudiantes en el proceso de creación de los especímenes, estos demuestran mayor sentido de responsabilidad, dedicación y autoconciencia, generando mayor compromiso, y entusiasmo con la clase y el proceso de aprendizaje. Adicionalmente, los especímenes producidos por ellos, serán de gran utilidad para clases a futuro.

Cardiovascular cast model fabrication and casting effectiveness evaluation in fetus with severe congenital heart disease or normal heart / 华中科技大学学报（医学）（英德文版）

To investigate the application and effectiveness of vascular corrosion technique in preparing fetal cardiovascular cast models, 10 normal fetal heart specimens with other congenital disease (control group) and 18 specimens with severe congenital heart disease (case group) from induced abortions were enrolled in this study from March 2013 to June 2015 in our hospital. Cast models were prepared by injecting casting material into vascular lumen to demonstrate real geometries of fetal cardiovascular system. Casting effectiveness was analyzed in terms of local anatomic structures and different anatomical levels (including overall level, atrioventricular and great vascular system, left-sided and right-sided heart), as well as different trimesters of pregnancy. In our study, all specimens were successfully casted. Casting effectiveness analysis of local anatomic structures showed a mean score from 1.90±1.45 to 3.60±0.52, without significant differences between case and control groups in most local anatomic structures except left ventricle, which had a higher score in control group (P=0.027). Inter-group comparison of casting effectiveness in different anatomical levels showed no significant differences between the two groups. Intra-group comparison also revealed undifferentiated casting effectiveness between atrioventricular and great vascular system, or left-sided and right-sided heart in corresponding group. Third-trimester group had a significantly higher perfusion score in great vascular system than second-trimester group (P=0.046), while the other anatomical levels displayed no such difference. Vascular corrosion technique can be successfully used in fabrication of fetal cardiovascular cast model. It is also a reliable method to demonstrate three-dimensional anatomy of severe congenital heart disease and normal heart in fetus.

Vasculatura portal intrahepática: descripción anatómica, radiológica y ecográfica / Portal intrahepatic vasculature: anatomical, radiological and sonographic description

El presente trabajo describe la arborización del sistema venoso portal intrahepático desde los enfoques anatómico, radiológico y ecográfico. Se realizaron 5 preparados anatómicos mediante inyección de resinas plásticas y posterior corrosión. Por radiología se contrastó el árbol portal en una pieza anatómica. Se realizaron estudios ecográficos bidimensionales y Doppler en 13 caninos de raza Beagle. Estos procedimientos permitieron a los autores comparar la tridimensionalidad de los preparados anatómicos con la bidimensionalidad de los estudios por imágenes.

This article describes the distribution of the intra-hepatic portal vein system by means of anatomy, radiology and ultrasound echo-scan approach. Five anatomical resin-embedded vein preparations with posterior corrosion have been done. The portal tree was depicted by radiology. Ultrasound B-mode and Doppler Scans were performed on 13 beagle dogs. These procedures allowed the authors to compare the three-dimensional preparations with the two-dimensional imaging methods.

Venous drainage from the tail of the pancreas to the lienal vein and its relationship with the distal splenorenal shunt selectivity / Drenagem venosa da cauda do pâncreas para a veia lienal e sua relação com a seletividade da anastomose esplenorrenal

PURPOSE: To identify the veins draining from the pancreatic tail to the lienal vein and its possible relationship with the loss of the distal splenorenal shunt selectivity. METHODS: Thirty eight human blocks including stomach, duodenum, spleen, colon and pancreas, removed from fresh corpses, were studied with the replenish and corrosion technique, using vinilic resin and posterior corrosion of the organic tissue with commercial hydrochloric acid, in order to study the lienal vein and its tributaries. RESULTS: The number of veins flowing directly to the splenic vein varied from seven to twenty two (14.52 ± 3.53). Pancreatic branches of the pancreatic tail flowing to the segmentary veins of the spleen were found in 25 of the anatomical pieces studied (65.79 percent). These branches varied from one to four, predominating one branch (60 percent) and two branches (24 percent). CONCLUSIONS: In 65.79 percent of the anatomical pieces studied, the veins of the pancreatic tail flowed in segmentary branches of the splenic vein. These branches could be responsible for the loss of distal splenorenal shunt selectivity. The complete disconnection of the pancreatic tail could increase the selectivity in this procedure.

OBJETIVO: Identificar as veias da cauda do pâncreas afluentes da veia lienal e a possível relação destes ramos com a perda de seletividade da derivação esplenorrenal distal. MÉTODOS: Foram estudadas 38 peças humanas, retiradas de cadáveres, contendo estômago, duodeno, baço, cólon e pâncreas, utilizando-se a técnica de repleção vascular com resina vinílica e posterior corrosão do tecido orgânico com o objetivo de se estudar o molde vascular da veia lienal e seus afluentes. RESULTADOS: O número de veias afluindo diretamente para a veia esplênica variou de sete a vinte dois (MA 14.52±3.53). Ramos pancreáticos da cauda do pâncreas afluindo para as veias segmentares do baço estavam presentes em 25 das peças estudadas (65,79 por cento). Estes ramos variaram de um a quatro, predominando um ramo (60 por cento) e dois ramos (24 por cento). CONCLUSÕES: Em 65,79 por cento das peças veias da cauda do pâncreas desembocavam em ramos segmentares da veia esplênica. Estes ramos poderiam ser responsáveis pela perda de seletividade da derivação esplenorrenal distal e a esqueletização completa da cauda do pâncreas poderia aumentar a seletividade neste procedimento.

[Study of the vascular system of bovine hind limb claw by means of corrosion casting method]

The importance of vessel structure has been shown in pathogenesis of the equine and bovine laminitis. Investigation on vessel structures is necessary to diagnose the mechanism of laminitis. Due to lack of literature on ovine digital arteries. Ovine digital vessels were studied using corrosion casting method. The hind limbs of 15 lambs of 3 months ages were separated from tarsus joint. After the dissection of dorsal metatarsal artery, normal saline and resin metacrylat were injected into the vessels. In this species, the dorsal common digital artery and common plantar digital artery have the main function in blood supply. One branch runs to the heel bulb and the other runs to the axial wall and sole. The third branch of these arteries is responsible to blood supply of periople tissue. The common dorsal digital artery and abaxial proper digital artery drain all venous blood from the claw. The dorsal proper digital artery arises from the numerous smaller veins which are joined to form the deep and superficial venous networks of coronary margin and wall. This vein delivers blood to the common dorsal digital artery. The axial proper plantar vein drained into the abaxial proper plantar vein. Two main vessels which arise from venous network of wall, sole and plantar surface of the bulb, drain into the abaxial proper plantar vein

Dominância coronariana em corações humanos em moldes por corrosão / Coronary dominance patterns in the human heart investigated by corrosion casting

Objetivo: Esse trabalho tem como objetivo analisar os padrões de dominância circulatória de corações humanos, o número de ramos que a artéria coronária direita fornece ao ventrículo esquerdo, o número de ramos que a artéria coronária esquerda fornece ao direito e a presença de anastomoses intercoronarianas, com sua localização e frequência. Métodos: Foram produzidos 25 moldes de corações submetidos à instilação de acrílico colorido e posterior corrosão com ácido clorídrico, no Laboratório de Cirurgia Experimental da FURB. Peças com lesões e cicatrizes não foram usadas. Resultados: Os corações pertenciam a indivíduos de ambos os sexos, sendo 17 (68 por cento) de indivíduos do sexo masculino, com idade média de 40,2 anos (15 a 70 anos). A dominância direita ocorreu em 18 (72 por cento) peças, com 1, 2, 3 e 4 ramos em 2, 14, 2 e 1 moldes, respectivamente; a dominância esquerda foi observada em 5 (20 por cento) casos, com 1 ramo em 4 moldes e 2 em 1 molde; e a dominância balanceada foi verificada em 2 (8 por cento) moldes. Houve diferença significativa entre a dominância direita e esquerda (α > 5 por cento), direita e balanceada (α > 5 por cento) e sem significância entre esquerda e balanceada (α < 5 por cento). Conclusão: A forma mais comum de circulação coronariana é a de dominância direita, com média de 2,16 ramos ao ventrículo esquerdo; enquanto nos casos de dominância esquerda, fornece em média 1,2 ramos. Não se observaram anastomoses intercoronarianas.

Objectives: The aim of this work was to analyze the dominance patterns of the circulation of the human heart, the number of branches from the right coronary artery to the left ventricle, the number of branches from the left coronary artery to the right ventricle and the frequency and location of intercoronary anastomoses. Methods: Casts were made of 25 hearts by the injection of colored acrylic resin and subsequent corrosion using hydrochloric acid at the experimental surgery laboratory of Furb. Specimens with lesions or scars were discarded. Results: The hearts, from both men (17 - 68 percent) and women (8 - 32 percent), had a mean age of 40.2 (15 to 70) years-old. Right dominance occurred in 18 (72 percent) subjects, with 1, 2, 3 and 4 branches leading to the left ventricle in 2, 14, 2 and 2 casts, respectively. Left dominance occurred in 5 (20 percent) with 1 branch leading to the right ventricle in 4 molds and 2 in one. Balanced circulation was observed in two molds (8 percent). There were significant differences between right and left dominance (α > 5 percent) and between right dominance and balanced circulation (α > 5 percent), however the same was not true between left dominance and balanced circulation (α < 5 percent). Conclusion: The most common form of coronary circulation is right dominance with an average of 2.16 branches leading to the left ventricle: when dominance is left, the average is 1.2 branches. No intercoronary anastomoses were observed.

Segmentos anátomo-cirúrgicos arteriais do rim de cutia (Dasyprocta prymnolopha) / Anatomical-surgical arterial segments of the kidney in agouti (Dasyprocta prymnolopha)

Foram estudados 20 pares de rins de cutias (Dasyprocta prymnolopha Wagler, 1831), com o objetivo de descrever os segmentos anátomo-cirúrgicos arteriais. As artérias renais foram injetadas com solução de Vinilite corada, e os rins foram submetidos à corrosão ácida para a obtenção dos moldes vasculares. Observou-se que as artérias renais da cutia, sempre únicas, dividiram-se em artéria setorial ventral e artéria setorial dorsal, caracterizando dois setores renais separados por plano avascular. As artérias setoriais penetraram no hilo renal (100 por cento dos casos). Estes vasos deram origem aos ramos segmentares responsáveis pela irrigação de territórios independentes em cada setor, os segmentos arteriais renais. No rim direito foram observados 3 (60 por cento), 4 (35 por cento) e 5 segmentos (5 por cento) no setor arterial ventral e 3 (30 por cento), 4 (45 por cento), 5 (20 por cento) e 6 (5 por cento) segmentos no setor dorsal e, à esquerda, 2(10 por cento), 3 (55 por cento) e 4 (35 por cento) segmentos no setor ventral e 3 (25 por cento), 4 (50 por cento) e 5 (25 por cento) no dorsal. Com base na distribuição arterial nos rins de cutia, observaram-se setores e segmentos arteriais independentes, sendo possível, desta forma, a realização de setoriectomia e segmentectomia nesta espécie.

Twenty pairs of agouti (Dasyprocta prymnolopha Wagler, 1831) kidneys were studied to describe the arterial anatomical-surgical segments. The renal arteries were injected with stained acetate vinyl, followed by procedures of acid corrosion in order to obtain vascular casts. It was found that the renal artery is always single and bifurcated into ventral and dorsal sectorial arteries. The sectorial arteries reached the kidneys (100 percent of the cases) through the hilus. These vessels gave origin to segmental branches responsible for kidney irrigation. At the right kidney, the ventral sectorial arteries gave origin to 3 (60 percent of the cases), 4 (35 percent) and 5 (5 percent) segmental branches; the dorsal sectorial arteries gave origin to 3 (30 percent), 4 (45 percent), 5 (20 percent) and 6 (5 percent) segmental arteries separated by a vascular sector. At the left kidney, the ventral sectorial arteries originated 2 (10 percent), 3 (55 percent) or 4 (35 percent) segmental branches; the dorsal sectorial arteries gave origin to 3 (25 percent), 4 (50 percent) and 5 (25 percent) segmental branches. Based on the arterial distribution of agouti kidneys, independent sections and arterial segments were found, so that it is possible to accomplish partial kidney resection surgery.

Métodos de análise quantitativa aplicados a modelos de corrosão vascular: revisão de literatura / Methods of quantitative analysis applied to vascular corrosion casts: a literature review

Os modelos de corrosão vascular propiciam um poderoso entendimento morfológico da microarquitetura dos vasos sangüíneos quando associados à microscopia eletrônica de varredura. Diversos sistemas de morfometria bidimensionais (2D) e tridimensionais (3D) têm sido propostos para a sua quantificação, bem como modelos matemáticos com o objetivo de entender os princípios ideais hemodinâmicos que governam estes sistemas circulatórios. Este artigo se propõe a realizar uma revisão de literatura desta evolução, desde as primeiras teses sobre os princípios ideais, fundamentais para o entendimento da hemodinâmica vascular, até o estado da arte dos sistemas de morfometria 3D.

Vascular corrosion casts combined with scanning electron microscopy provide apowerful morphologic understanding of the vascular microarchitecture. Several bidimensional(2D) and threedimensional (3D) systems, as well as mathematical models have been proposed aiming at the quantification of vascular models, in order to study the hemodynamic optimality principles ruling those circulatory systems. This paper aims to accomplish a literature review of this evolution from the foremost thesis about optimality principles, vital to the knowledge of vascular hemodynamics, up to the state-of-the-art 3D morphometry systems.

Emprego da técnica de modelo de corrosão vascular em pesquisa odontológica / Employment of the technique of vascular corrosion model in dental investigation

A técnica de modelo de corrosão vascular produz a réplica dos leitos vasculares de tecidos normais ou patológicos. Uma vez associada à microscopia eletrônica de varredura (MEV), essa técnica fornece detalhes do arranjo anatômico tridimensional da vascularização, tais como o diamêtro vascular, a impressão das células endoteliais e dos seus núcleos na superficie do modelo vascular e o trajeto das arteríolas e vênulas que compoõem a estrutura avaliada. A imagem tridimensional da réplica vascular proporcionada pelo MEV é uma das vantagens do método. O presente estudo teve por objetivo descrever a técnica de modelo de corrosão vascular, discutir suas vantagens e desvantagens, bem como exemplificar o seu emprego na pesquisa odontológica.

Réplicas vasculares da língua de ratos Wistar com resina Marcox: implicações da corrosão / Vascular replicas of wistar rats´tongues with Mercox resin: implications of corrosion

O método de corrosão vascular com resina Merox-CL-2B para visualização sob microscopia eletrônica de verredura (MEV) permite que se realize uma análise da angioarquitetura. Para tanto, há necessidade de maceração parcial ou total do tecido orgânico que se encontra ao redor do molde. Com relação à corrosão, cada órgão a ser analisado apresenta especificidades técnicas, relacionadas ao tamanho e ao tipo de tecido que forma uma espécime. Há necessidade de se eliminarem duas camadas na superficie dorsal da língua de ratos antes de expor o molde com resina. Existem poucos protocolos padronizados com relação à maceração de línguas. Dessa forma, neste trabalho foram utilizados nove ratos machos Wistar adultos, com dois meses de idade e aproximadamente 200g de peso, manipulados sob anestesia intraperitoneal para realização da técnica de réplica vascular com resina acrílica. A partir daí, três línguas foram maceradas com concentrações de hidróxido de sódio (NaOH) a 1,10 e 20 por cento com temperatura padronizada em 45°, por 10, 24 e 36h, respectivamente. Todos osmoldes obtidos foram submetidos à secagem pelo ponto-crítico e colocados para metalização om duas camadas de carbono, seguidas por uma camada de ouro. Quando utilizadas soluções a 10 e 20 por cento para maceração, foi possivel notar a formação de estruturas que dificultam esse processo, assim como depósitos sobre as amostras, tornando-as extremamente sujas. Um período de 24 e 36h é necessário para remoção da ceratina da superfície dorsal da língua de ratos com solução de NaOH a 1 por cento nas condições deste estudo.

Scanning electron microscopy of angioarchitecture of palatine gingiva in young rabbits / Microscopia eletrônica de varredura da angioarquitetura da gengiva palatina de coelhos jovens

A angioarquitetura da gengiva palatina de coelhos jovens foi estudada utilizando-se o método de modelos de corrosão vascular. Os modelos de corrosão foram obtidos a partir da injeção de resina de baixa viscosidade (Mercox« Cl-2B) sendo, posteriormente observados ao microscópio eletrônico de varredura (MEV). A gengiva palatina apresentou áreas de diferentes disposições vasculares. Os capilares da gengiva palatina e da papila interdental emitiam diversas projeções, não detectadas, porém, no sulco gengival, onde a rede capilar mostrava-se de arranjo irregular, aplainado. Os vasos sangüíneos, nutrindo o osso alveolar, também foram observados

Técnica para preparo angioarquitetônico hepático de ratos / Preparation technique for angioarquitetonic liver model in rats

Os métodos de injeção-corrosão são os principais métodos utilizados para o estudo da anatomia vascular do fígado. Alguns autores mencionam a técnica para estudo de fígado de cães, porcos, hamsters, coelhos e gatos, entretanto são escassos os trabalhos que mencionam o estudo da anatomia hepática de ratos. Desta forma é importante o conhecimento de novas técnicas de preparo para modelos angioarquitetônico de fígados, possibilitando um melhor conhecimento da anatomia e por conseguinte, aperfeiçoando significativamente a abordagem operatória deste órgão. Em função disso o objetivo do presente estudo é demonstrar a técnica para realização de angioarquitetura venosa do fígado de ratos. Foram utilizados 10 Rattus norvegicus albinus (Wistar), com massa corporal entre 250 e 300g, para verificar a funcionalidade do método. A técnica para preparo de modelo vascular apresenta três tempos fundamentais: cateterização da veia porta, veia cava intra-abdominal e veia cava intra-torácica; preparo e infusão da solução de acrílico; corrosão em ácido clorídrico e maceração da peça. Concluímos que, esta técnica é factível e apresenta como vantagem um baixo custo (30 dólares) e com a utilização de duas cores diferentes de tinta pode-se separar o sistema vascular portal do sistema de drenagem supra-hepático, portanto sendo capaz de moldar a estrutura vascular hepática de ratos.

Palatine mucosa angioarchitecture study of young rabbits using scanning electron microscopy

Pocos trabajos han relatado la angioarquitectura de animales jóvenes. Este estudio contribuye con las investigaciones acerca de la distribución tridimensional de la mucosa palatina en conejos jóvenes usando MEB. La corrosión vascular del molde fue obtenida usando resina de baja densidad-Mercox© CL-2B. No pudimos observar redes específicas en la cúpula de los pliegues palatinos, en los interpliegues y mucosa labial. Fueron observados solamente loops capilares y pliegues transversos palatinos

Angiogenesis in Distraction Osteogenesis / 대한정형외과학회잡지

PURPOSE: Distraction osteogenesis is now a standard method for bone lengthening. However, little is known about the exact mechanism of new bone formation. This study was undertaken to investigate the temporal and spatial changes of angiogenesis during the periods of distraction osteogenesis; the three-dimensional microarchitecture of newly formed vessels and their origin; and the role of angiogenesis with reference to mineralization. MATERIALS AND METHODS: Sprague-Dawley rat's tibia was osteotomized subperiosteally and was distracted at a rate of 0.5 mm per day for two weeks after one week of latency period. Vascular corrosion casting using Mercox was done on the hindlimbs before osteotomy, and at the 1st, 2nd, 3rd, 4th, and 6th weeks postoperatively. Replicated microvascular structures and their distribution patterns at the distraction site and adjacent parent bone were observed temporally and spatially under scanning electron microscope. These findings were compared with radiographical and histological observations. RESULTS: 1. At postoperative first week and second week, proliferation of periosteal vessels were more pronounced than that of endosteal vessels, which corresponded to the early new bone formation in subperiosteal area on histologic sections. 2. At postoperative third week, arterial branches from the medulla of parent bone entered into the distraction gap. Multiple longitudinal vascular branches sprouted out from the newly formed vascular network, and ran towards the interzone. 3. At postoperative fourth week, there was vascular proliferation in the periosteal side of interzone, from which branches arose towards the parent bone. 4. At postoperative sixth week, vascular networks of both sides connected to each other, and few angiogenetic findings were observed. CONCLUSIONS: In distraction osteogenesis, angiogenesis occurred actively during the distraction period and then gradually decreased with time. Taken together with radiographical and histological findings, close temporal and spatial relationship between the angiogenesis and new bone formation at the distraction site was revealed.

An experimental study on the vascular changes of rat molar periodontal ligament following orthodontic tooth movement using vascular corrosion casting method / 대한치과교정학회지

This study was undertaken to investigate the three dimensional vascular changes of periodontal ligament following orthodontic tooth movement. Experimental tooth movement was carried out in 96 Sprague-Dawley rats with the weight of 250g. They were divided into four experimental groups (each 24 rats). The left maxillary first molar was moved mesially with 25g force in group I, and with 75g force in group II. Each three animals were sacrificed after 1, 6, 12, 24 hours, and 3, 7, 14, 21 days. In group III, 25g mesial force was applied for 3 days, and in group IV, 75g mesial force was applied for 3 days. Then the appliances were removed, and each three animals were sacrificed after 1, 6, 12, 24 hours, and 3, 7, 14, 21 days from removal of appliance. The contralateral molars were used for control group. Casting media was injected via left ventricle and polymerized in warm water. After corrosion of surrounding soft tissue, three dimensional vascular changes were examined using scanning electron microscopy. The findings of this study were as follows: 1. Pressure side of group I and II showed degenerative vascular changes such as vascular compression, reduction of vasculature, leakage of casting media. But, regenerative changes were dominant after 7 days of tooth movement. Although the degenerative vascular changes were more severe in group II, which was exposed to heavy force, the timing of these changes was not different between two groups. 2. Periodontal vasculature was reestablished by the growth of new capillaries and their differentiation and union from the remaining periodontal vessels and vessels of alveolar bone marrow. Although vascular regeneration was more rapid in group I, which was exposed to light force, the vasculature was not fully normalized in both groups even after 21 days. 3. There was no remarkable changes in tension side of group I and II, but looping of capillary, new capillary growth, dilation of vessels, redirection of vessels in the direction of tensile force were occurred. 4. In pressure side of group III and IV, in which appliance was removed after 3 days of orthodontic force, bone resorption was continued even after removal of appliance. Regeneration of vasculature was initiated after 1-6 hours, and it was more rapid in group III than group IV. In both groups, the vasculature was not fully normalized even after 21 days. 5. After removal of appliance, tension side of group III and IV showed vascular compression and loss of vasculature.

Three dimensional structures of pulmonary elastin; airway vs vascular elastin

Elastin is known to occur in the lung parenchyma and pleura as well as in the pulmonary vessels, but no detailed studies of this elastin's linkage between them have been done in three dimensions. For many years we have known that there is abundant elastin in the mammalian lungs, which may be associated with etiology of causing emphysema. We have developed selective casting methods to allow us to determine the location where elastin is found morphologically. The method involves casting either the vasculature via the right ventricle, or the airways via the trachea in the air sacs. Studies of the vasculature were done with the lung inflated to 80% of the vital capacity. The casted lungs were then put in 0.1 N NaOH at 75 degrees C for 48 hours, turning them frequently. THis method removed all non-elastin tissues. The scanning electron microscopy (SEM) was used to reveal the three dimensional pictures of elastin structures from both lung parenchyma and pulmonary vessels. Elastin was seen as fenestrated sheets and some fibers in both the vessels and the airways. Elastin in the two different locations was often interconnected. Studies on 6 dogs, 8 rabbits, and 2 pigs showed no significant species difference at the level of resolution of the SEM, which was used to study the specimens after they had been freeze-dried.

Morphological Characteristics of the Atrium as an Endocrine Organ: I. Luminal Structure of Atria in Various Animals Using Corrosion Casting Method / 체질인류학회지

The internal morphology of the right and left atria of rabbit, cat, pig, and human, particularly on the luminal structures of the auricle, were observed by stereomicroscope improving corrosion casting method using latex. Structures of the right auricular casts markedly differ from those of the left side, consisting of defined surface sturctures with distinct folds and branching patterns. The main atrial region consists of smooth surfaced single mass of the latex, and the auricular region is characterized by well developed branching patte and delicate small folds. The results of this study indicate that auricula have well organized duct-like luminal structures and many small pouches (out-pocketings) expanding the internal wall of the duct-like structures and small pouches. The endocardial nuclear imprints on the surface of the folds in the auricular region appeared larger and deeper than those on the atrium proper region of the atrial cast. These morphological observation will be aid in understanding structures of auricular lumen, regional differences of the endocardium and their function to the secretory process of atrial natriuretic peptide (ANP) release.

Scanning electron microscopic findings of the rat femoral head vascular architecture using a corrosion casting / 대한정형외과학회잡지

No abstract available.

Anteroir Uveal Vasculature in Rabbit Eye with Scanning Electron Microscopy

Using ocularluminal methylmethacrylate corrosion casting, we obsesved three-dimentional anterior uveal microcirculation of the rabbit eyes with scanning electron microscope. The two terminal branches of each long posterior ciliary artery turn opposite direction within the iris to run concentric to the pupil; the superior and inferior branches forming the major iridic circle (MIC). Radial branches run both proximally and distally from the MIC, the former distributing to the ciliary process, choroid and ciliary body and the latter distributing to the iris. Venous drainage of the anterior uvea is taken care of by the large iridociliary veins and by marginal capillaries of the process. Each of these drains to the large vessel layer of the choroid and subsquently to the vortex vein.